Rebel Janata Dal (United) leaders Sharad Yadav and Ali Anwar on Monday was disqualified from Rajya Sabha.
Upper house chairman Venkaiah Naidu in his communication said they cease to be Rajya Sabha members with immediate effect.
The development comes after the Election Commission recognized the Nitish-led JD(U) faction as the real party and entitled it to use the reserved 'arrow' poll symbol.
The JDU had sought their disqualification on the grounds that they had attended a rally of opposition parties in Patna in violation of its direction.
The former JD(U) president, who is at present in Gujarat to campaign against the BJP, is expected to respond to his disqualification tomorrow.
Yadav and Anwar were among a few elected representatives who denounced Kumar for joining forces with the BJP and forming a coalition government in Bihar which, they said, was against the mandate given by people in the Assembly polls.
OneIndia News (with PTI inputs)