"I announce this clearly that threats against Iranian nation is worthless and childish, since our nation resisted different kinds of enemy's threats over the past 35 years and emerged victorious," Xinhua quoted Rouhani as saying.
The Iranian president urged Western countries to change their rhetoric in dealing with Iran, arguing that the language of threat would lead to solidarity among Iranians and unity against the world powers.
He denounced sanction pressures against his country "as oppressive, illegal and wrong", saying that the West can no longer continue these sanctions against Iran.
"In the recent talks, we struck the first blow on the sanctions regime," said Rouhani.
The president's remarks were followed by an applause from the large crowd gathered in capital Tehran's Azadi square.