Named the 'She-ros Cafe', it is run by survivors of acid attacks, Chanchal, Ritu, Rupa and Geeta, on a 'pay what you wish' basis.
"The initiative is part of the 'Stop Acid Attack' campaign," said Alok Dixit, the founder of the coffee shop.
Similar cafes are to be shortly opened in Kanpur and Delhi.
The Agra hangout is supported by local NGO Chaanv.
Sunday evening saw Bundelkhand's Gulabi Gang leader Sampat Pal cut the ceremonial cake to announce the cafe's opening.
Talking to reporters, Sampat Pal said acid attackers should be publicly thrashed.