The firm in a statement said, "Alibaba Group has communicated to Yahoo! that Yahoo's statement that it is 'aligned' with the position Google took last week was reckless given the lack of facts in evidence."
Last week, Yahoo had said it stood aligned with Google that attacks on company networks are deeply disturbing and violations of Internet user privacy is something that must be opposed.
This is after Google had threatened to pull out from China over censorship and cyber attacks.
Alibaba Group had a testy relationship with Yahoo ever since the departure of Yahoo's former chief executive Jerry Yang.
Yahoo folded its search business and invested $1 billion in Alibaba Group in 2005 in exchange for a 40 percent stake.
The US search giant sold its stake in Alibaba.com late in 2009.
The latest statement from Alibaba highlights the growing friction between the two sides.