"It's inadmissable that Brazil, the country with the largest black population out of Africa, has racism issues," Rousseff said.
Last week's incident came a day after racist chants were directed at referee Marcio Chagas da Silva during the Rio de Janeiro state championship match between Esportivo and Veranopolis.
Rousseff also pledged to make the anti-racism message a theme of this year's World Cup.
"We are going to take on racism. We have agreed with the UN and FIFA that this will be a World Cup against racism."