Coach Luiz Felipe Scolari has the luxury of being able to field the same team that destroyed world champions Spain 3-0 in the final of last year's Confederations Cup, with Barcelona star Neymar the focal point of a powerful and settled line-up.
The five-time champions are expected to qualify from their group without too much trouble but Barcelona defender Dani Alves admitted there was anxiety ahead of the Sao Paulo opener, as they seek to ease the jitters.
"I have always said that if you don't feel anxiety it is not worth being a professional athlete. The opening game is difficult, important," said Alves.
"The three points count but so does the idea of sending out a message to our rivals. The most important game at the World Cup, for everyone, is the opening game."
He added: "We will only know on Thursday if everything is OK. We are very confident and we want this moment to come. We want to enjoy the World Cup. We are going to try to give a good image."
The only question mark for tournament favourites Brazil for the match at the new Corinthians Arena would appear to be over the out-of-sorts Oscar, with his impressive Chelsea team-mate Willian pushing for a starting berth.
But Scolari, who masterminded Brazil's last World Cup win in 2002, is likely to stick with his tried-and-tested winning formula against 18th-ranked Croatia after the same 11 edged past Serbia 1-0 in a friendly last week, courtesy of a goal from Fred.
Brazilian reserve striker Jo, speaking after training yesterday, said the mood in the camp was calm but the nerves would be jangling as kick-off approached.
"At this moment, everyone is calm," said the striker. "When we go to Sao Paulo and the game approaches, there will be excitement, anxiety. But the opening of the World Cup in our country will be different to others."