Police visited the suite number 345 at Leela Palace hotel in south Delhi where former Union minister Shashi Tharoor's wife Sunanda was mysteriously found dead on January 17 this year.
Police revealed that they found fluid marks on the bed and the carpet, and broken glass.
The investigation of the crime scene took place after the AIIMS medical board in its final opinion reiterated its earlier stand that Sunanda had died of poisoning.
"They found a broken glass from the crime scene besides collecting other evidence from there. The fresh evidence will be examined by the CFSL and its report will be submitted to the police at the earliest," said a police officer.
The findings of the medical board had pointed to the presence of poison in her viscera. The reports said the residue of alprax tablets found in her was non-fatal, ruling out overdose of sleeping pills.
The police had earlier said there were traces of urine on Sunanda's bed but had not disclosed anything about traces of fluid on the carpet.
The suite was kept sealed all these months and was opened for the first time since the initial police investigation.
Earlier, Yahoo and Google had provided information regarding Pushkar's email and chat conversations with various persons on these platforms, along with IP addresses.
Based on the information, police has made a list of the people with whom the wife of former Union minister Shashi Tharoor had communicated and who may now be quizzed in this regard.
Sunanda Pushkar, 52, was found dead in a mysterious condition in her room at a five-star hotel in south Delhi on January 17.