Out of the five women, only two are ranked in the top 50, with the highest ranked being Jindal Group's Savitri Jindal at 14th place ($4.9 billion).
Together, the 100 richest Indians net-worth stood at $259 billion, while the combined wealth of the five richest women stood at 8.83 billion, accounting for just 3.4 per cent, US-based business magazine Forbes said.
Savitri Jindal is the richest women in India with a net worth of $4.9 billion. She is ranked 14 in the list, which is topped by Reliance Industries Chairman Mukesh Ambani with a fortune of $21 billion.
Ms Jindal, who has been the chairperson of the O P Jindal Group since her husband Om Prakash's death in 2005, saw her fortune decline by close to $10 billion since 2010, amid continuing problems plaguing OP Jindal Group.
"Company halted work in August at two power plants being built in eastern India at cost of $2 billion, following death threats from Maoist rebels. Son Sajjan reportedly plans to sell US steel mill to reduce debt at JSW Steel," Forbes said.
Ms Jindal is followed by Bennett, Coleman & Co's Chairperson Indu Jain, who is ranked 29th with a net worth of $1.9 billion.
Anu Aga of Thermax was ranked 86th on the list with a net worth of $730 million, while Biocon's Kiran Mazumdar Shaw, with a fortune of $655 million was ranked 96 on the list.
Referring to Ms Shaw, Forbes said she is the "wealthiest self-made woman in India".
Science magazine recently ranked Biocon at No. 6 among the best 20 global biotech employers, ahead of Novo Nordisk and Novartis. An active philanthropist, she recently donated $5 million for a new research unit at her cancer hospital in Bangalore, Forbes said.
Besides, Shobhana Bhartia of the Hindustan Times was ranked 98th on the list with a net-worth of $645 million.
Ms Bhartia chairs and runs listed HT Media, publisher of English daily Hindustan Times, Hindi daily Hindustan and business paper Mint.