"I was grieved to learn about the demise of your beloved wife Smt Kamla Advani," the AIADMK supremo said in a condolence letter sent to the BJP leader.
"Although no words of solace would really be adequate in such circumstances, I wish to share your grief by conveying my deepest condolences to you and to all the other members of your family," she said.
Jayalalithaa said she prayed that the Almighty "may give you the strength and fortitude to withstand this personal sorrow. May her soul rest in peace."
Kamla Advani died at the All India Institute of Medical Sciences (AIIMS) in Delhi yesterday due to heart failure.