This was the ninth regular bail application of Asaram to be rejected. An interim bail plea was rejected by the high court last month.
Prosecution counsel P C Solanki said the court also rejected the argument of defence that the trial of the case was taking long.
A teenage girl had accused Asaram of sexual assault at his ashram in Manai village near Jodhpur. The girl belonging to Shahjahanpur in Uttar Pradesh was a student at the ashram.
Following her complaint against Asaram, he was arrested by Jodhpur Police on 31 August, 2013 and has been in jail since then.