The manoeuvre started at 2:10 am on Saturday and raised the spacecraft's apogee from 40,186 km to 71,636 km with a burn time of 707 seconds, ISRO said.
The space agency had performed the first two manoeuvres in the last couple of days, in the series of five scheduled on the Mars mission.
The fourth and fifth operations would be performed on 11 and 16 November to raise the apogee to 1,00,000 km and 1,92,000 km, respectively.
After the successful completion of these operations, the mission is expected to take on the "crucial event" of the trans-Mars injection around 12.42 am on 1 December.
ISRO's PSLV C 25 successfully injected the 1,350-kg Mangalyaan Orbiter (spacecraft) into orbit around Earth about 44 minutes after a textbook launch at 2.38 pm from Satish Dhawan Space Centre at Sriharikota on Tuesday, marking the successful completion of the first stage of the Rs450-crore mission.