The German side have an away goals advantage after coming back to draw 1-1 in the first leg in Insanbul, but Galatasaray will be hoping to continue their impressive record away from home and complete a hat-trick of wins.
However, Schalke have lost just twice in 16 home games since 2010-11, while Galatasaray have an impressive record on their travels which reads W9 D6 L2 since 2008.
Schalke reached the quarter-finals of the Champions League in 2011 while Galatasaray reached the last eight back in 2001.
The Bundeliga side have been rocked following the injury to Klaas-Jan Huntelaar, who was stretchered off against Borussia Dortmund after colliding with his own keeper on Saturday.
Jermiane Jones is suspended and manager Jens Keller will have to deal with the injury list of Kyriakos Papadopoulos, Ibrahim Afellay and Ciprian Marica. Teemy Pukki is likely to replace Huntelaar upfront while Marco Hoger will be in for suspended Jones.
Meanwhile, Didier Drogba could return to the starting line-up after being benched in the 1-0 loss to Genclerbirligi. Tomas Ujfalusi is out injured with Sabri Sarioglu or Emmanuel Eboue to fit in at right back.
*Schalke have won their last three games and remain unbeaten in the Champions League this season.
*Galatasaray won two away games against CFR Cluj and Braga this campaign.
Schalke (4-2-3-1): Hildebrand-Uchida, Howedes, Matip, Kolasinac-Neustadter, Hoger-Farfan, Draxler, Bastos-Pukki
Galatasaray (4-2-3-1): Muslera-Sabri, Semih, Nounkeu, Riera-Hamit, Melo, Selcuk-Sneijder-Burak, Drogba