Victory in itself would not guarantee the Greeks a place in the last eight -- even if group leaders Russia defeat Poland in the day's other match -- but it would end Czech hopes of progressing to the quarter-finals.
Greece's never-say-die attitude is reminiscent of their style at Euro 2004, when they effectively ground their more flamboyant opponents into submission. But it has been strengthened by a desire to deliver some much-needed joy to their hard-pressed compatriots back home, who are wilting under horrendous financial hardship.
The Czechs by contrast will be looking to bounce back from their opening 4-1 drubbing by Russia and also avenge their Euro 2004 semi-final defeat by the Greeks, who shocked everyone by going on to win the tournament.
The Greeks will be without their first-choice central defensive pairing of Sokratis Papastathopoulos, who is suspended after his harsh sending-off in the Poland match, and Avraam Papadopoulos, who has torn cruciate ligaments. But, whilst the lively Czech attack will be hoping to exploit defensive weaknesses, the Greek forwards, too, will be looking to expose their opponents' defensive failings, which were brutally exposed by the Russians.
In some quarters, the Greeks were criticised for letting three points slip from their grasp, as captain Giorgos Karagounis had a penalty saved. Experienced striker Giorgos Samaras, though, said such is the spirit in the camp that the roof could cave in and they would still summon up the spirit to dig themselves out.
"We never give up and because we never give up, we don't like to lose games. And that's something you cannot buy or find. It's all about the mentality in the dressing room," said the Celtic striker.