Mayor Ghulam Haider Hamidi, who was 65 years old, had been mentioned as a person to replace President Hamid Karzai's powerful half brother, gunned down July 12 in his heavily fortified home in Kandahar by a close associate.
The assassination of Ahmed Wali Karzai set off a power struggle in Kandahar and raised doubts about the strength of the president's support in the south as well as the stability of the critical area for the US-led war effort.
Five days later, Karzai's inner circle suffered another blow when gunmen strapped with explosives killed Jan Mohammad Khan, an adviser to the president on tribal issues and a former governor of Uruzgan province in the south. A member of parliament also was killed in the July 17 attack at Khan's home in Kabul.
Wednesday's blast took place at a government building in the heart of Kandahar city.
Zalmai Ayubi, spokesman for Kandahar provincial governor, said one civilian also was killed and another civilian and a security guard were wounded in the explosion.
"The suicide bomber put explosives inside his turban," said Sher Shah Yousafzai, deputy police chief in Kandahar province.
"As soon as the mayor came to work, the attacker detonated his explosives." Bismullah Afghan Mal, a member of the upper house of parliament from Kandahar, confirmed the mayor's death.
Kandahar police officials said Hamidi traveled yesterday to a district in the city where there was a dispute about the construction of houses. They said the attacker infiltrated the group that came today to talk to the mayor about that issue.