Clijsters has pulled out due to a recurrence of the ankle problem, that has dogged her for the last few weeks.
In a statement, Clijsters said, "I'm very, very disappointed to have to withdraw from Wimbledon after injuring my foot again at the tournament in s'Hertogenbosch. At this moment I feel frustrated that it has to happen now before one of my favourite tournaments."
And added, "I've always enjoyed being a part of the Wimbledon atmosphere but I have no other choice now but to rest, recover and to not play tennis for a few weeks."
The Belgian was the second seed for the tournament, which is likely to see the comeback of the Williams sisters - Serena and Venus after a long injury-enforced hiatus. Her absence will elevate Vera Zvonareva, the Wimbledon 2010 runners-up to the second seed. China's Li Na , Belarus' Victoria Azarenka and Russia's Maria Sharapova will be the third, fourth and fifth seed respectively.
Clijsters, who has never won an Wimbledon title, despite a glorious career underwent more tests on the ankle-injury and scans showed that it will need some rest to heal properly.
In the wake of Serena Williams' comeback, there was a growing anticipation of a match between the duo in the grass-courts of Wimbledon, making it the ultimate battle in contemporary women's tennis. However, it might have to wait a bit more.