The joint registrar of the court had earlier aksed Tiwari, Rohit Shekar and Ujjwala Sharma (Rohit Shekar's mother) to appear in the court's dispensary to give their blood samples for the DNA test.
The court had turned down the plea made by 85-year-old Tiwari on Dec 23, last year. The court said that it was the right of the child to know who their biological parents were.
The Delhi High Court, on Nov 12, last year rejected Tiwari's plea which sought deletion of some paragraphs from a paternity suit filed against him by Rohit Shekar.
On May 10, last year the apex court rejected Tiwari's plea to judge the paternity suit. The High Court also dismissed his plea that said the petition against him was filed by Shekhar, 31 years after he was born to bring a bad name to him.
Tiwari quit as the governor of Andhra Pradesh after there were allegations against him that hinted at his connection in a sex scandal in the Raj Bhavan.