Justice Gita Mittal directed Tiwari to comply with the court's August 13, 2010 order and pay the amount to Shekhar by May 10 before the court's joint registrar.
While dismissing Tiwari's plea for deletion of some portions of Shekhar's paternity suit, the court had imposed a cost of Rs 75,000 and had directed him to pay the amount. He had challenged the court's order before a division bench.
On November 12 last year, the division bench too had dismissed 84-year-old Tiwari's plea challenging the single judge order to pay the amount.
The former Andhra Pradesh Governor had contended that the high court's single-judge bench had erred in rejecting his plea and imposing the cost as it was done without issuing a notice to the other party.
Tiwari had sought deletion of a paragraph from the suit in which Shekhar had alleged that the veteran leader avoided meeting him at the Delhi Airport when he made an attempt to do so in 2005.
Tiwari, who has denied having any physical relation with the petitioner's mother, has also been ordered by the High Court to undergo a DNA test for determination of Shekhar's paternity.
The court has sought the help of a Delhi government dispensary for determination of modalities to draw blood samples of Tiwari, Shekhar and his mother for the DNA test.