Scientists produce mice from 2 fathers using stem cell technology

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Washington, Dec 9 (ANI): Reproductive scientists at the M.D. Anderson Cancer Center, Texas have produced male and female mice from two fathers using stem cell technology.

The achievement of two-father offspring in a species of mammal could be a step toward preserving endangered species, improving livestock breeds, and advancing human assisted reproductive technology (ART).

It also opens the provocative possibility of same-sex couples having their own genetic children, the researchers note.

The team led by Richard R. Berhringer manipulated fibroblasts from a male (XY) mouse fetus to produce an induced pluripotent stem (iPS) cell line.

About one percent of iPS cell colonies grown from this XY cell line spontaneously lost the Y chromosome, resulting in XO cells. The XO iPS cells were injected into blastocysts from donor female mice.

The treated blastocysts were transplanted into surrogate mothers, which gave birth to female XO/XX chimeras having one X chromosome from the original male mouse fibroblast.

The female chimeras, carrying oocytes derived from the XO cells, were mated with normal male mice. Some of the offspring were male and female mice that had genetic contributions from two fathers.

"Our study exploits iPS cell technologies to combine the alleles from two males to generate male and female progeny, i.e. a new form of mammalian reproduction," said the authors.

In the future, it may also be possible to generate human oocytes from male iPS cells in vitro. Used in conjunction with in vitro fertilization, this would eliminate the need for female XO/XX chimeras, although a surrogate mother would still be needed to carry the two-father pregnancy to term.

Using a variation of the iPS technique, the researchers say "it may also be possible to generate sperm from a female donor and produce viable male and female progeny with two mothers."

The study was posted at the online site of the journal Biology of Reproduction. (ANI)

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