Washington, Dec 07 (ANI): A new research has developed a method, which could significantly reduce the amount of irradiation needed to kill 99.99 percent of Salmonella, E.coli and other pathogens on fresh produce.
A team of Texas AgriLife Research engineers found that by packing produce in a Mylar bag filled with pure oxygen and her colleagues found they could almost half the amount of radiation needed to kill those pathogens.
Reducing the amount of radiation is not so much a safety measure as it is a way to preserve quality of the produce, said Carmen Gomes, AgriLife research food safety engineer.
The U.S. Food and Drug Administration recently approved the use of irradiation at dosages of up to 4,000 Gray on leafy greens such as spinach, Gomes said.
A Gray is a measure of ionizing radiation dose and it is equal to the absorption of 1 Joule of ionizing radiation by 1 kilogram of matter.
"That dosage was determined as what was necessary to achieve an 100,000-fold reduction of such pathogens as E. coli O157:H7 and salmonella. However, we know based on previous research conducted by our group that above 1 kilo Gray (1,000 Gray) the quality of leafy vegetables starts to decay and they lose their freshness," noted Gomes.
A 100,000-fold reduction corresponds to a 99.999 percent kill rate, according to Dr. Rosana Moreira, another member of the team.
"If you had 100,000 bacteria in your vegetable, it means you would end up with just one bacteria still living," Moreira said.
The team was looking for ways to reduce the amount of ionizing radiation without reducing its effectiveness in killing pathogens. An electron-beam gun is typically used to irradiate food for several reasons, Gomes said.
Electron beams will kill pathogens without using a radioactive material for a source. But electron beams also generate ozone, a corrosive form of oxygen, which is a drawback for many applications. However, ozone has a bactericidal function as well.
Working with this concept, the researchers packed the vegetables in Mylar bags filled with pure oxygen, a nitrogen oxygen mix or plain air. Prior to bagging, the team uniformly inoculated both fresh and frozen spinach samples with a cocktail containing either salmonella or listeria cultures. They then subjected the sample bags to various levels of radiation, ranging from 0.2 to 1.25 kilo Gray.
After irradiation, the team tested the samples for pathogens. Their test showed modified packaging containing either pure oxygen or the nitrogen/oxygen mix increased the sensitivity of salmonella or listeria to radiation without changing the way the radiation affected the vegetables, Gomes said.
"So, there is no harm done at all. This allows the processor to irradiate the produce at lower doses and still kill the pathogens. The good thing about this is that product quality is assured," concluded Gomes. (ANI)