Supporters and opponents of a separate Telangana state stopped trains and buses even as an all-party meeting on Telangana issue took place in New Delhi.
Railway authorities cancelled 89 trains as a precaution while state-owned Road Transport Corp (RTC) partially suspended bus services.
Police arrested a large number of protesters, including leaders of political parties and student groups resorting to transport blockades in the three regions.
Dozens of trains were stopped by anti-Telangana protesters in Kadapa district of Rayalaseema and in Guntur and Krishna districts of coastal Andhra.
A Joint Action Committee (JAC) of political parties and student groups have called for 'rail roko' to protest the talks convened by home minister P Chidambaram.