New approach to check toxic genes in disease-causing bacteria devised

Washington, Oct 10 : After studying a bacterium called Photorhabdus asymbiotica, scientists at Universities of Bath and Exeter have developed a new method, called Rapid Virulence Annotation (RVA), for checking toxic genes in disease-causing bacteria which infect insects and humans.

The results of the study could in the future lead to new vaccines and anti-bacterial drugs.

Photorhabdus asymbiotica, normally infects and kills insects, but can also cause an unpleasant infection in humans.

The researchers tested groups of genes from the bacteria against three types of invertebrates (insects, worms and amoebae) and mammalian cells.

They then identified toxins and other molecules, called virulence factors, made by the bacteria that allow it to infect each type of organism.

After pointing out the genes responsible for each of these possible virulence factors and comparing them with the genes of well known bacteria, the scientists have been able to map out which regions of the bacteria's DNA control its ability to infect and damage invertebrates, and also potentially humans. "Many bacteria have evolved to infect one particular type of plant or animal and most of the toxins they use to do this also have an effect in other hosts," said Dr Nick Waterfield from the University of Bath's Department of Biology and Biochemistry.

He added: "Some of the toxins they use for infecting can also allow the bacteria to jump across into another species like humans, perhaps with fatal consequences."

Dr Maria Sanchez-Contreras, who works with Dr Waterfield at the University of Bath said: "We have developed a new way of discovering a greater number of previously unknown toxins and measuring how dangerous or virulent these bacteria are. Identifying the genes responsible for the production and secretion of these bacterial toxins will allow us find ways to prevent disease.

"Our new technique, called Rapid Virulence Annotation (RVA), allows us to make faster assessments of the disease-causing agents in multiple types of organism; it lets us pinpoint sequences of genes which may pose a risk to humans; and it gives us a powerful tool to identify virulence genes in other known bacteria. Finally, it helps us identify new targets for drugs to fight these diseases and control pests, and for developing preventive vaccines."

Richard ffrench-Constant, Professor of insect microbiology from the University of Exeter's Cornwall Campus added: "RVA allows us to look for virulence factors that are totally novel and does not rely upon traditional searches based on factors already known from other bacteria. We have already discovered that some totally unexpected genes are indeed involved in bacterial virulence. This technique should prove to be a gold mine for potential vaccine candidates."

Already, researchers are using this relatively cheap and highly accurate RVA technique in other disease-causing bacteria to identify the genes, which allow some diseases to jump the species barrier.

The findings of the study have been published in the prestigious journal Proceedings of the National Academy of Sciences of the United States of America.

ANI