Scientists identify DNA 'barcode' gene for plants

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Washington, Feb 6 : Scientists at the Imperial College London's Department of Life Sciences and the Royal Botanic Gardens, Kew, in collaboration with researchers in Costa Rica and South Africa, have identified a 'barcode' gene that can be used to distinguish between the majority of plant species on Earth.

According to the research team, led by Dr Vincent Savolainen of the Imperial College London, the gene could lead to new ways of easily cataloguing different types of plants in species-rich areas like rainforests.

It could also help to protect the world's plants as they argue that it may lead to accurate methods for identifying plant ingredients in powdered substances, such as traditional Chinese medicines, and to help monitor and prevent the illegal transportation of endangered plant species, they added.

The researchers found that DNA sequences of the gene matK differ among plant species but are nearly identical in plants of the same species.

This means that the matK gene can provide scientists with an easy way of distinguishing between different plants, even closely related species that may look the same to the human eye.

The researchers made this discovery by analysing the DNA from different plant species. They found that when one plant species was closely related to another, differences were usually detected in the matK DNA.

The team carried out two large-scale field studies: one on the exceptionally diverse species of orchids found in the tropical forests of Costa Rica, and the other on the trees and shrubs of the Kruger National Park in South Africa. Dr Savolainen and his colleagues in the UK worked alongside collaborators from the Universities of Johannesburg and Costa Rica who played a key role in this new discovery.

Using specimens collected from Costa Rica, Dr Savolainen and colleagues were able to use the matK gene to identify 1,600 species of orchid. In the course of this work, they discovered that what was previously assumed to be one species of orchid was actually two distinct species that live on different slopes of the mountains and have differently shaped flowers adapted for different pollinating insects.

In South Africa the team was able to use the matK gene to identify the trees and shrubs of the Kruger National Park, also well known for its big game animals.

Dr Savolainen explains that in the long run the aim is to build on the genetic information his team gathered from Costa Rica and South Africa to create a genetic database of the matK DNA of as many plant species as possible, so that samples can be compared to this database and different species accurately identified.

"In the future we'd like to see this idea of reading plants' genetic barcodes translated into a portable device that can be taken into any environment, which can quickly and easily analyse any plant sample's matK DNA and compare it to a vast database of information, allowing almost instantaneous identification, " he said.

Although Dr Savolainen concedes that such technological applications may be some years away from realisation, he says the potential uses of the matK gene are substantial.

"There are so many circumstances in which traditional taxonomic identification of plant species is not practical - whether it be at ports and airports to check if species are being transported illegally, or places like Costa Rica where the sheer richness of one group of plants, like orchids, makes accurate cataloguing difficult," he said.

The study appears in the Proceedings of the National Academy of Sciences.

ANI

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